So many things

This week has truly been interesting . I found out that it is possible for a person to have two different blood types. It happens most often with twins exchanging genetic material in vitro and is commonly called chimerism. After receiving a letter from United Blood Services after a recent donation I was made aware that I may be one of these people. The letter also stated that they are not sure which blood I could be given if I ever required a transfusion because according to the test I have A and O blood. . I also am not a twin so this information was even more confusing. I decided to delve more into this and began by typing my blood. The test showed little to no agglutination in any of the serums. I called my mother to try to help me fill in the blanks. She stated that a fetus was removed through a D&C 27 months after my birth. After much research I learned that chimerism can be the result from one twin absorbing the genetic material from a nonviable fraternal twin fetus. I explained the situation and she confirmed that when I was 5 I had a minor surgery and she was told that I had a rare blood type and could not receive a transfusion if the need arises. She also confirmed that I was a very selfish child so the possibility of me absorbing a twin is highly probable. I would like to test my DNA to see if this is truly the case. I also want to mix different blood types with mine on a microscope slide in order to see if there is anyone that is compatible with me. A few brave souls volunteered this week but so far none of the RBC's got along with mine. I am currently looking for primers in order to get a final answer out of my DNA. 

PROTEINS:
With the help of Josh and his mad hot gun skills I was finally able to successfully pour an agarose vertical gel and it was really cool. I loaded to 50 mL syringes with smelly SDS agarose gel and loaded the vertical plate while Josh kept the glass warm with a heat gun. I am still trying to figure out the best way to place the gel and the proper voltage and running time for the vertical chamber before I perform an electrophoresis with this. I am also waiting on bio-markers before I run and electrophoresis with the denser PAGE gels.